Keep your cells warm outside the incubator, avoid the edge effect and use more wells.
Eppendorf Cell Culture Plates are designed for expansion of smaller cell numbers and for cell-based assays.
The chimney-well design avoids inhomogeneous growth of cells in the outer ring of wells which reduces costs and increases efficiency. If cells need to spend more time outside of the incubator, filling the inter-well spaces of the plate will keep the temperature stable and prevent unwanted temperature shifts. We optimized the optical performance by enhancement of the planarity, material clarity, and reduction of the liquid meniscus to facilitate both manual and automated read-out.
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